AutologIX – Autologous Exosomes

AutologIX is a protocol for obtaining a concentrate of autologous exosomes from platelet-rich plasma (PRP) derived from the patient’s own blood. This is made possible by a ready-to-use system composed of the ProtSmart6 ultraconcentrator and Idria G tubes. It is the unique tested system for the extraction and ultraconcentration of exosomes.

Two key medical devices are involved:

PROTSMART6
A Class IIa medical device – a selective exosome concentrator using ultrafiltration technology with special filters containing hundreds of pores. The filter has a permeability of approximately ⏈15,000 KDa, with a perfusion diameter of less than 5 nm. Thanks to its ability to perform selective concentration and ultrafiltration, water and salt ion fragments are expelled into a waste bag, while exosomes, proteins, and cells are retained inside the filter with a specialised membrane. The membrane’s high filtration capacity is due to its structure, as its internal wall features a high density of small pores.

IDRIA G
Two Class IIB medical-grade tubes – a medical device designed to prepare an autologous platelet-rich plasma concentrate from the patient’s blood. During centrifugation with Idria G, vesicles are released and remain suspended in the plasma.

The set also includes:
– A butterfly needle with holder
– 2 x 20 ml syringes

To correctly perform the procedure, a horizontal centrifuge is essential. It must provide the appropriate centrifugal force and optimal spin parameters – enabling the extraction of intact microvesicles that release exosomes at high concentrations while preserving their full regenerative potential. We also offer such a centrifuge in our product range.

What are exosomes and how do they work?

Autologous exosomes are used in various fields of medicine, including orthopaedics, dermatology, pain management, wound healing, hair loss therapy, and rejuvenation treatments. What gives them their regenerative and reparative properties?

Exosomes are extracellular vesicles (EVs) surrounded by a phospholipid bilayer, released into the extracellular space. They carry nucleic acids, proteins, and lipids from the cells they originate from. Exosomes deliver this cargo to recipient cells, acting as mediators of intercellular communication in both physiological and pathological conditions. They also serve as vehicles for transporting genetic material between cells.

They have the ability to transmit critical intercellular information. During inflammation, they stimulate the immune response and exhibit antibacterial activity. It is also known that once absorbed by stem cells, exosomes release their contents into the cells, significantly influencing the metabolic dynamics of stem cells and enhancing their regenerative potential.

Indications:

• Skin rejuvenation with improvement in tone, texture, elasticity, and uniformity of the dermal layer
• Reduction of wrinkles and filling of fine lines
• Revitalisation of the eye contour
• Rejuvenation of the skin on the hands, face, and neck
• Accelerated healing of the skin following more invasive procedures, e.g. surgery
• Treatment of hair loss – including alopecia areata and androgenetic alopecia, as well as support during hair transplantation
• Reduction of inflammation

Importantly, in aesthetic indications, just one treatment can deliver long-lasting effects, due to the prolonged influence of PLT-Exos on the metabolism of local cells (including MSCs and ASCs). The procedure is repeated no sooner than approximately 9 to 16 months later! In hair loss therapy, only two sessions are performed, spaced 2–3 weeks apart.